Cannabidiol (CBD) is a popular natural remedy used in many pharmaceutical, food, and cosmetic products. CBD is just one of over 100 chemical compounds found in the cannabis plant. Unlike tetrahydrocannabinol (THC), CBD is not psychoactive. This characteristic makes CBD an appealing option for those who are looking for relief from pain and other symptoms without the mind-altering effects associated with consuming marijuana or resin concentrates. CBD oil is made by extracting the compound from the plant, then diluting it with a carrier oil (e.g., coconut or hemp seed oil).

The standard HPLC method requires 45 minutes to perform by highly trained analysts. In contrast to the primary method, Vis-NIR spectroscopy is a cost-efficient and fast analytical solution for the determination of cannabinoid content in oils.

All 17 measured Vis-NIR spectra (Figure 2) were used to create a prediction model for quantification of the cannabinoid content. The quality of the prediction models was evaluated using cross-validation, which display a very high correlation between Vis-NIR prediction and primary method values. The respective figures of merit (FOM) display the expected performance of a prediction during routine analysis.

This application note demonstrates the feasibility of the DS2500 Liquid Analyzer for the determination of cannabinoid content in CBD oils. In comparison to the HPLC method (Table 3), the time to result is a major advantage of NIR spectroscopy, since a single measurement is performed within one minute.